Sample Submission Guidelines
Licensed marijuana businesses will need to follow testing requirements in 4-110 – Regulated Marijuana Testing Program: Sampling Procedures. Our sample submission guidelines will assist with understanding the Sample Size Requirements.
Which Tests are Required?
For Remediated Concentrates.
* See footnote
|Pressurized Metered Dose Inhalers||
If made with Solvent-based Concentrate.
|Vaporizor Delivery Devices||
If made with Solvent-based Concentrate.
|Industrial Hemp Products|
* Audited Products require Total aerobic microbial count, Staphylococcus Aureus, Pseudomonas aeruginosa, Bile tolerant gram negative bacteria, and Candida albicans.”
“Audited Product” means a Regulated Marijuana Product with an intended use of: (1) metered dose nasal spray, (2) vaginal administration, or (3) rectal administration. Audited Product types may raise public health concerns requiring additional safeguards and oversight. These product types may only be manufactured and Transferred by a Medical Marijuana Products Manufacturer in strict compliance with Rule 5-325 or Retail Marijuana Products Manufacturer in strict compliance with Rule 6-325. Prior to the first Transfer of an Audited Product to a Medical Marijuana Store, Medical Marijuana Cultivation Facility that has a Centralized Distribution Permit, Retail Marijuana Store or Retail Marijuana Cultivation Facility that has obtained a Centralized Distribution Permit, the Medical Marijuana Products Manufacturer or Retail Marijuana Products Manufacturer shall submit to the Division and, if applicable, to the Local Licensing Authority or Local Jurisdiction an independent third-party audit verifying compliance with Rule 5-325 or Rule 6-325. All rules regarding Regulated Marijuana Product apply to Audited Product except where Rules 5-325, 6-325, 4-115, 3-1010, and 3-1015 apply different requirements.
“Pressurized Metered Dose Inhaler” means inhalable Regulated Marijuana Concentrate, which may be comprised of other Ingredients, and a pressurized propellant inside a device that administers a dose of an aerosolized composition.
“Vaporizer Delivery Device” means inhalable Regulated Marijuana Concentrate, which may be comprised of other Ingredients inside a device that uses a heating element to create a vapor including, but not limited to, vaporizer cartridges and vaporizer pens.
Sample Size Requirements
|Harvest Batch Size||Sampling Size||Test Batch Size|
|≤ 10 pounds||8 separate 0.5 gram Samples||4 grams|
|10.01 – 20 pounds||12 separate 0.5 gram Samples||6 grams|
|20.01 – 30 pounds||15 separate 0.5 gram Samples||7.5 grams|
|30.01 – 40 pounds||18 separate 0.5 gram Samples||9 grams|
|40.01 – 100 pounds||23 separate 0.5 gram Samples||11.5 grams|
|> 100 pounds||29 separate 0.5 gram Samples||14.5 grams|
Regulated Marijuana Concentrate
|Production Batch Size||Sampling Size||Test Batch Size|
|≤ 1 pound||8 separate 0.25 gram Samples||2 grams|
|1.01 – 2 pounds||12 separate 0.25 gram Samples||3 grams|
|2.01 – 3 pounds||15 separate 0.25 gram Samples||3.75 grams|
|3.01 – 4 pounds||18 separate 0.25 gram Samples||4.5 grams|
|4.01 – 10 pounds||23 separate 0.25 gram Samples||5.75 grams|
|> 10 pounds||29 separate 0.25 gram Samples||7.25 grams|
Regulated Marijuana Product
|Production Batch Size||Sample Size||Test Batch Size|
|1 – 100 products||2 separate Samples||1|
|101 – 500 products||4 separate Samples||1|
|501 – 1000 products||6 separate Samples||1|
|1,001 – 5,000 products||8 separate Samples||1|
|5,001 – 10,000 products||10 separate Samples||1|
|> 10,000 products||12 separate Samples||1|
4-110 – Regulated Marijuana Testing Program: Sampling Procedures – page 175
Samples must be submitted in final packaging.
Industrial Hemp Sample Size Requirements
Absent sampling and testing standards established by the Colorado Department of Public Health and Environment for the sampling and testing of Industrial Hemp Product, a Person Transferring an Industrial Hemp Product to a Licensee pursuant to the Marijuana Code and these Rules shall comply with the sampling and testing standards set forth in these 4-100 Series Rules – Regulated Marijuana Testing Program and as required by these Rules.
On November 27, 2019, the Colorado Marijuana Enforcement Division (MED) issued a Compliance Tip for industrial hemp product sales and use as an ingredient. This tip clarifies Colorado Revised Statutes 44-10-103, 44-10-601, and Rules 5-105, 6-105, 5-305, 6-305.
We encourage all industry stakeholders to read the compliance tip to understand industrial hemp product sales and use as an ingredient.
Submitting your Samples
The CDHPE Reference Library’s sample collection protocols must be used for the collection of marijuana/marijuana product samples submitted to marijuana testing facilities for required testing in accordance with 4-110 – Regulated Marijuana Testing Program: Sampling Procedures
Agricor Minimum Sample Sizes
Below are the absolute minimum sample sizes that we require to perform our analytical testing services. Use these amounts when submitting samples for R & D testing.
|Test Types||Agricor Minimum Sample Size|
|Homogeneity||4 servings with label claims|
|Residual Solvents (RSA)||1 gram|
|Microbial Contamination||3 grams|
|Pesticides||2 grams (enough to perform 3 tests)|
|Heavy Metals||1 gram|
|pH||1 unit or no less than 20 mL|
|aW||1 unit or no less than 2 grams|
|Density||20 mL or 20 grams|
Combining Required Tests
You may elect to submit each Test Batch separately, but the MED also gives you the option of combining multiple tests into one Test Batch, which you can submit using one METRC tag.
MED Industry Bulletin 17-09 states:
The Samples collected may be composited into a single physical package and submitted as a single Test Batch. That single Test Batch may be submitted for multiple required tests.
If you plan on combining multiple Test Types into one Test Package, the sample size will need to meet our minimum sample size requirments outlined above and to follow the minimum sample size requirements outlined in 4-110 – Regulated Marijuana Testing Program: Sampling Procedures.
|Combined Test Types||Agricor Minimum Sample Size|
|Potency / RSA||2 grams|
|Potency / Terpenes||2 grams|
|Micro / Homogeneity||4 grams / 4 servings|
|Potency / Pesticides||3 grams|
|Potency / Micro||4 grams|
|Micro / Pesticides||5 grams|
|Potency / RSA / Pesticides||4 grams|
|Micro / Potency / RSA||5 grams|
|Potency / Micro / Pesticides||6 grams|
|Potency / Micro / Pesticides / Heavy Metals||7 grams|